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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Methodology of rearing of viruliferous population of aphid Rhopalosiphum padi, as a vector of Barley yellow dwarf virus (BYDV)
 Štolcová, Jindra ; Červená, Zuzana ; Bártová, Šárka ; Chrpová, Jana ; Kumar, Jiban
The methodology describes innovative procedures of rearing virulent population of Rhopalosiphum padi aphids, as a vector of Barley yellow dwarf virus (BYDV), for artificial infections of cereals. The method is a modification of existing procedure used in our laboratory for this purpose. The advantage of present methodology includes more abundance of viruliferous aphids within shorter period and effective control against aphid predators than the earlier used methodology.
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Methodics of molecular detection of Barley yellow dwarf virus in vectors by RT-PCR
 Jarošová, Jana ; Jaňourová, Blanka ; Kumar, Jiban
Barley yellow dwarf is one of the most important viral diseases of cereals in the world. It can cause significant yield losses in most cereal species, including wheat, barley, rice, corn, oat and rye. In this methodology we provide assays for reverse transcription - polymerase chain reaction (RT-PCR) for sensitive detection of barley yellow dwarf virus directly in the vectors, in aphids. This way it is possible to evaluate infectivity of vectors and from that conclude subsequent measures. The methodology is meant to serve the employees of State Phytosanitary Administration.
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